Optimized real-time fluorescence PCR assay for the detection of porcine Circovirus type 3 (PCV3)

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Development of real-time PCR assay for detection of porcine circovirus-like virus P1 in domestic pigs in China

BACKGROUND The porcine circovirus-like agent P1 is a newly discovered DNA virus with a single-stranded circular genome that is highly homologous to that of porcine circovirus type 2. P1 infection can cause symptoms resembling postweaning multisystemic wasting syndrome. This study aims to develop a rapid, sensitive and specific method to detect P1. RESULTS A pair of primers was designed and us...

متن کامل

Quantitative detection of chicken meat routine mislabeling in emulsion type sausages and burgers by SYBR green real time PCR assay

ABSTRACT- Today, the authenticity of meat products with less costly and desirable species has increased. Therefore and considering religious, economicalor public health concerns, proper actions should be taken to prevent such frauds. In this study, real time PCR assay was applied for rapid, sensitive and specific identification and quantification of chicken tissue in meat products. Specific pri...

متن کامل

Development and validation of a SYBR green real-time PCR for the quantification of porcine circovirus

not available 687 Krakowka, S., Ringler, S.S., Arumugam, P. , McKillen, J., McIntosh, K., Hartunian, C., Hamberg, A., Rings, M., Allan, G., Ellis, J.A. (2008) Evaluation of Mycoplasma hyopneumoniae bacterins for porcine torque teno virus DNAs American Journal of Veterinary Research, 69, 1601-1607 Objective-TO determine whether commercial Mycoplasma hyopneumoniae bacterins sold for use in swine ...

متن کامل

Detection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay

Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples wer...

متن کامل

Rapid Detection of Porcine Circovirus Type 2 by TaqMan-based Real-time Polymerase Chain Reaction Assays

PCV2 is the primary causative agent of porcine circovirus-associated disease (PCVAD). In this study, a TaqMan-based real-time polymerase chain reaction (PCR) assay targeting ORF2 gene of PCV2 was developed and their sensitivities and specificities were investigated. The results indicated that the standard curve had a wide dynamic range (102-107copies/μL) with a linear correlation (R2) of 0.997 ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: BMC Veterinary Research

سال: 2020

ISSN: 1746-6148

DOI: 10.1186/s12917-020-02435-y